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BIOLOGY AND BIOMEDICAL SCIENCES (L41)  (Dept. Info)Arts & Sciences  (Policies)SP2025

L41 BIOL 4522Laboratory in Protein Analysis, Proteomics, and Protein Structure3.0 UnitsLab Required
Description: This laboratory class is structured around a biochemical question of relevance to the research community. Students will design and generate mutants of an enzyme to test hypotheses about its mechanism and kinetics. The course consists of three sections: molecular biology, protein biochemistry, and structural biology. In the first section, students the learn the principles of DNA manipulation, cloning, mutagenesis, and DNA sequencing and apply them to design and generate mutant constructs of the protein of interest. In the second section, students learn the principles of heterologous expression of proteins in bacteria, protein purification, SDS-PAGE, protein quantification, and kinetic analysis of enzyme activity. These methods are applied to test the students' hypotheses by assaying the activity of their mutant enzymes. The final section of the course introduces students to concepts of structural biology including protein crystallization, x-ray diffraction, and computer modeling of protein structures. Fulfills the upper-level laboratory requirement for the Biology major. Prerequisites: Chem 262 and either Bio 451 or Bio 4810/Chem 481. Suggested to be taken concurrently with Bio4820/Chem482. Enrollment limit is set at zero. Due to high demand for this course, please fill out this survey at this link: https://wustl.az1.qualtrics.com/jfe/form/SV_ermrbGP0bgxmMXY. The department will manage registration for this course based on this information. The department will manage registration for this course based on this information. Graduating seniors who need an upper-level laboratory to complete requirements of a biology major program have priority.
Attributes:A&S IQNSMArchNSMArtNSMBUSCI
Instruction Type:Classroom instruction Grade Options:CP Fees:
Course Type:HomeSame As:N/AFrequency:Annually / History
SecDays       TimeBuilding / RoomInstructorFinal ExamSeatsEnrollWaits
01----F--1:00P-1:50PLife Sciences / 311 CruzNo Final0230
Actions:BooksSyllabus
Syllabi are provided to students to support their course planning; refer to the syllabus for constraints on use.
Waits Not Allowed
AM-W----1:00P-3:50PJolley / 3101 CruzNo Final0230
Actions:BooksSyllabus
Syllabi are provided to students to support their course planning; refer to the syllabus for constraints on use.
Waits Not Allowed
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Home/Ident

A course may be either a “Home” course or an “Ident” course.

A “Home” course is a course that is created, maintained and “owned” by one academic department (aka the “Home” department). The “Home” department is primarily responsible for the decision making and logistical support for the course and instructor.

An “Ident” course is the exact same course as the “Home” (i.e. same instructor, same class time, etc), but is simply being offered to students through another department for purposes of registering under a different department and course number.

Students should, whenever possible, register for their courses under the department number toward which they intend to count the course. For example, an AFAS major should register for the course "Africa: Peoples and Cultures" under its Ident number, L90 306B, whereas an Anthropology major should register for the same course under its Home number, L48 306B.

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Q=ME Q (Medical School)

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