WUSTL Course Listings Login with WUSTL Key
Search Results: Help Display: Open + Closed     Just Open     Just Closed View: Regular     Condensed     Expanded
1 course found.
BIOLOGY AND BIOMEDICAL SCIENCES (L41)  (Dept. Info)Arts & Sciences  (Policies)SP2021

L41 BIOL 4522Laboratory in Protein Analysis, Proteomics, and Protein Structure3.0 UnitsLab Required
Description:This laboratory class is structured around a biochemical question of relevance to the research community. Students will design and generate mutants of an enzyme to test hypotheses about its mechanism and kinetics. The course consists of three sections: molecular biology, protein biochemistry, and structural biology. In the first section, students the learn the principles of DNA manipulation, cloning, mutagenesis, and DNA sequencing and apply them to design and generate mutant constructs of the protein of interest. In the second section, students learn the principles of heterologous expression of proteins in bacteria, protein purification, SDS-PAGE, protein quantification, and kinetic analysis of enzyme activity. These methods are applied to test the students' hypotheses by assaying the activity of their mutant enzymes. The final section of the course introduces students to concepts of structural biology including protein crystallization, x-ray diffraction, and computer modeling of protein structures. Fulfills the upper-level laboratory requirement for the Biology major. Prerequisites: Chem 262 and either Bio 451 or Bio 4810/Chem 481. Suggested to be taken concurrently with Bio4820/Chem482. Enrollment limit is set at zero, and students are enrolled from the waitlist. Graduating seniors who need an upper-level laboratory to complete requirements of a biology major program have priority.
Attributes:A&S IQNSMArchNSMArtNSMBUSCI
Instruction Type:Classroom instruction Grade Options:CP Fees:
Course Type:HomeSame As:N/AFrequency:Annually / History
SecDays       TimeBuilding / RoomInstructorFinal ExamSeatsEnrollWaits
01----F--1:00P-1:50PRemote / LA Cruz, JezNo Final0110
Desc:Remote/Synchronous each meeting
REG-DelayStart: 1/25/2021   End: 5/13/2021
Actions:BooksSyllabus
Syllabi are provided to students to support their course planning; refer to the syllabus for constraints on use.
Waits managed by dept.
AM-W----1:00P-3:50PRebstock / 112 Cruz, JezNo Final0110
Desc:Classroom Instruction/Synchronous each meeting The Laboratory section of this course will be held in either Rebstock 112 or Rebstock 118. Professor Cruz will assing the groups.
REG-DelayStart: 1/25/2021   End: 5/13/2021
Actions:BooksSyllabus
Syllabi are provided to students to support their course planning; refer to the syllabus for constraints on use.
Waits managed by dept.
Label

Home/Ident

A course may be either a “Home” course or an “Ident” course.

A “Home” course is a course that is created, maintained and “owned” by one academic department (aka the “Home” department). The “Home” department is primarily responsible for the decision making and logistical support for the course and instructor.

An “Ident” course is the exact same course as the “Home” (i.e. same instructor, same class time, etc), but is simply being offered to students through another department for purposes of registering under a different department and course number.

Students should, whenever possible, register for their courses under the department number toward which they intend to count the course. For example, an AFAS major should register for the course "Africa: Peoples and Cultures" under its Ident number, L90 306B, whereas an Anthropology major should register for the same course under its Home number, L48 306B.

Grade Options
C=Credit (letter grade)
P=Pass/Fail
A=Audit
U=Satisfactory/Unsatisfactory
S=Special Audit
Q=ME Q (Medical School)

Please note: not all grade options assigned to a course are available to all students, based on prime school and/or division. Please contact the student support services area in your school or program with questions.